汉氏菱形藻生理特性对硝基苯胁迫的响应(3)
【作者】网站采编
【关键词】
【摘要】高质量浓度的硝基苯使汉氏菱形藻细胞膜受到的损伤加剧,其选择透过性增大导致细胞的电解质外泄加剧,致使其伤害率上升,以至于在测试时间内细胞没
高质量浓度的硝基苯使汉氏菱形藻细胞膜受到的损伤加剧,其选择透过性增大导致细胞的电解质外泄加剧,致使其伤害率上升,以至于在测试时间内细胞没有恢复正常的生理迹象。
4 结论
(1)汉氏菱形藻暴露在质量浓度为100 mg/L硝基苯环境下1~6 d内,对藻细胞的伤害是很难恢复的,即导致细胞死亡。(2)硝基苯质量浓度为10、50 mg/L时,处理后1 d汉氏菱形藻的可溶性蛋白含量降至最低点,2 d时的可溶性糖含量降到最低点,之后恢复到稳定值,这种变化趋势反映了汉氏菱形藻对硝基苯胁迫下的响应机制。(3)通过对硝基苯胁迫下汉氏菱形藻细胞生长、可溶性糖含量、可溶性蛋白含量、叶绿素a含量、电导率等生理生化响应模式研究,结果表明10、50 mg/L硝基苯胁迫下影响汉氏菱形藻细胞的正常生理态势,经响应后可以逐步恢复到正常生理生长状态,而100 mg/L硝基苯胁迫下,对藻细胞的伤害是难以恢复的。
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